8aa9 | pdb_00008aa9

Crystal structure of the Rpa1 AROD-OB-1 domains

spinqualitylabelsall models 8aa9, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8aa9 is a 2 chain structure with sequence from Pyrococcus abyssi GE5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CL, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G8ZHS0_PYRAB Publication Abstract from PubMed Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential roles in DNA replication, recombination and repair. Little is known about the structure of RPA in Archaea, the third domain of life. By using an integrative structural, biochemical and biophysical approach, we extensively characterize RPA from Pyrococcus abyssi in the presence and absence of DNA. The obtained X-ray and cryo-EM structures reveal that the trimerization core and interactions promoting RPA clustering on ssDNA are shared between archaea and eukaryotes. However, we also identified a helical domain named AROD (Acidic Rpa1 OB-binding Domain), and showed that, in Archaea, RPA forms an unanticipated tetrameric supercomplex in the absence of DNA. The four RPA molecules clustered within the tetramer could efficiently coat and protect stretches of ssDNA created by the advancing replisome. Finally, our results provide insights into the evolution of this primordial replication factor in eukaryotes. DNA-binding mechanism and evolution of replication protein A.,Madru C, Martinez-Carranza M, Laurent S, Alberti AC, Chevreuil M, Raynal B, Haouz A, Le Meur RA, Delarue M, Henneke G, Flament D, Krupovic M, Legrand P, Sauguet L Nat Commun. 2023 Apr 22;14(1):2326. doi: 10.1038/s41467-023-38048-w. PMID:37087464 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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