6xmg | pdb_00006xmg

Cryo-EM structure of Cas12g ternary complex

spinqualitylabelsall models 6xmg, resolution 4.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6xmg is a 3 chain structure with sequence from Lachnospiraceae bacterium ND2006 and Metagenome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Cas12g, the type V-G CRISPR-Cas effector, is an RNA-guided ribonuclease that targets single-stranded RNA substrate. The CRISPR-Cas12g system offers a potential platform for transcriptome engineering and diagnostic applications. We determined the structures of Cas12g-guide RNA complexes in the absence and presence of target RNA by cryo-EM to a resolution of 3.1 A and 4.8 A, respectively. Cas12g adopts a bilobed structure with miniature REC2 and Nuc domains, whereas the guide RNAs fold into a flipped 'F' shape, which is primarily recognized by the REC lobe. Target RNA and the CRISPR RNA (crRNA) guide form a duplex that inserts into the central cavity between the REC and NUC lobes, inducing conformational changes in both lobes to activate Cas12g. The structural insights would facilitate the development of Cas12g-based applications. Cryo-EM structure of the RNA-guided ribonuclease Cas12g.,Li Z, Zhang H, Xiao R, Han R, Chang L Nat Chem Biol. 2021 Apr;17(4):387-393. doi: 10.1038/s41589-020-00721-2. Epub 2021 , Jan 25. PMID:33495647 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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