8btk | pdb_00008btk

Structure of the TRAP complex with the Sec translocon and a translating ribosome

spinqualitylabelsall models 8btk, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8btk is a 10 chain structure with sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 1MA, 4AC, 5F0, 5MC, 5MU, 6MZ, A2M, AAC, AME, AYA, B8N, G7M, GTP, HIC, HY3, M3L, MA6, MG, MLZ, NMM, OMC, OMG, OMU, PSU, SAC, SPD, SPM, UR3, UY1, V5N, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A5F9C4D3_RABIT A0A5F9D5B2_RABIT Publication Abstract from PubMed The translocon-associated protein (TRAP) complex resides in the endoplasmic reticulum (ER) membrane and interacts with the Sec translocon and the ribosome to facilitate biogenesis of secretory and membrane proteins. TRAP plays a key role in the secretion of many hormones, including insulin. Here we reveal the molecular architecture of the mammalian TRAP complex and how it engages the translating ribosome associated with Sec61 translocon on the ER membrane. The TRAP complex is anchored to the ribosome via a long tether and its position is further stabilized by a finger-like loop. This positions a cradle-like lumenal domain of TRAP below the translocon for interactions with translocated nascent chains. Our structure-guided TRAP mutations in Caenorhabditis elegans lead to growth deficits associated with increased ER stress and defects in protein hormone secretion. These findings elucidate the molecular basis of the TRAP complex in the biogenesis and translocation of proteins at the ER. Molecular basis of the TRAP complex function in ER protein biogenesis.,Jaskolowski M, Jomaa A, Gamerdinger M, Shrestha S, Leibundgut M, Deuerling E, Ban N Nat Struct Mol Biol. 2023 May 11. doi: 10.1038/s41594-023-00990-0. PMID:37170030 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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