8ai9 | pdb_00008ai9
S10T variant of glutathione transferase Chi 1 from Synechocystis sp. PCC 6803 in complex with glutathione
Structural highlights
Publication Abstract from PubMedGlutathione transferases (GSTs) constitute a widespread superfamily of enzymes notably involved in detoxification processes and/or in specialized metabolism. In the cyanobacterium Synechocsytis sp. PCC 6803, SynGSTC1, a chi-class GST (GSTC), is thought to participate in the detoxification process of methylglyoxal, a toxic by-product of cellular metabolism. A comparative genomic analysis showed that GSTCs were present in all orders of cyanobacteria with the exception of the basal order Gloeobacterales. These enzymes were also detected in some marine and freshwater noncyanobacterial bacteria, probably as a result of horizontal gene transfer events. GSTCs were shorter of about 30 residues compared to most cytosolic GSTs and had a well-conserved SRAS motif in the active site ((10)SRAS(13) in SynGSTC1). The crystal structure of SynGSTC1 in complex with glutathione adopted the canonical GST fold with a very open active site because the alpha4 and alpha5 helices were exceptionally short. A transferred multipolar electron-density analysis allowed a fine description of the solved structure. Unexpectedly, Ser10 did not have an electrostatic influence on glutathione as usually observed in serinyl-GSTs. The S10A variant was only slightly less efficient than the wild-type and molecular dynamics simulations suggested that S10 was a stabilizer of the protein backbone rather than an anchor site for glutathione. Biochemical and Structural Characterization of Chi-Class Glutathione Transferases: A Snapshot on the Glutathione Transferase Encoded by sll0067 Gene in the Cyanobacterium Synechocystis sp. Strain PCC 6803.,Mocchetti E, Morette L, Mulliert G, Mathiot S, Guillot B, Dehez F, Chauvat F, Cassier-Chauvat C, Brochier-Armanet C, Didierjean C, Hecker A Biomolecules. 2022 Oct 13;12(10):1466. doi: 10.3390/biom12101466. PMID:36291676[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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