8oit | pdb_00008oit
39S human mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA
Structural highlights
DiseaseRM12_HUMAN The disease may be caused by variants affecting the gene represented in this entry. FunctionRM12_HUMAN As a component of the mitochondrial large ribosomal subunit, it plays a role in mitochondrial translation (PubMed:23603806). Associates with mitochondrial RNA polymerase to activate transcription.[1] [2] Publication Abstract from PubMedThe genetic code that specifies the identity of amino acids incorporated into proteins during protein synthesis is almost universally conserved. Mitochondrial genomes feature deviations from the standard genetic code, including the reassignment of two arginine codons to stop codons. The protein required for translation termination at these noncanonical stop codons to release the newly synthesized polypeptides is not currently known. In this study, we used gene editing and ribosomal profiling in combination with cryo-electron microscopy to establish that mitochondrial release factor 1 (mtRF1) detects noncanonical stop codons in human mitochondria by a previously unknown mechanism of codon recognition. We discovered that binding of mtRF1 to the decoding center of the ribosome stabilizes a highly unusual conformation in the messenger RNA in which the ribosomal RNA participates in specific recognition of the noncanonical stop codons. Molecular basis of translation termination at noncanonical stop codons in human mitochondria.,Saurer M, Leibundgut M, Nadimpalli HP, Scaiola A, Schonhut T, Lee RG, Siira SJ, Rackham O, Dreos R, Lenarcic T, Kummer E, Gatfield D, Filipovska A, Ban N Science. 2023 May 5;380(6644):531-536. doi: 10.1126/science.adf9890. Epub 2023 , May 4. PMID:37141370[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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