2n7f | pdb_00002n7f

NMR solution structure of muO-conotoxin MfVIA

spinqualitylabelsall models 2n7f Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2n7f is a 1 chain structure with sequence from Synthetic construct. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The muO-conotoxins MrVIA, MrVIB and MfVIA inhibit the voltage-gated sodium channel NaV1.8, a well-described target for the treatment of pain; however, little is known about the residues or structural elements that define this activity. In this study, we determined the three-dimensional structure of MfVIA, examined its membrane-binding properties, performed alanine-scanning mutagenesis and identified residues important for its activity at human NaV1.8. A second round of mutations resulted in [E5K,E8K]MfVIA, a double mutant with greater positive surface charge and greater affinity for lipid membranes compared to MfVIA. This analogue had increased potency at NaV1.8 and was analgesic in the mouse formalin assay. Development of a muO-conotoxin Analogue with Improved Lipid Membrane Interactions and Potency for the Analgesic Target NaV1.8.,Deuis JR, Dekan Z, Inserra MC, Lee TH, Aguilar MI, Craik DJ, Lewis RJ, Alewood PF, Mobli M, Schroeder CI, Henriques ST, Vetter I J Biol Chem. 2016 Mar 29. pii: jbc.M116.721662. PMID:27026701 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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