5unk | pdb_00005unk

NMR structure of the RED subdomain of the Sleeping Beauty transposase

spinqualitylabelsall models 5unk Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5unk is a 1 chain structure with sequence from Oncorhynchus mykiss. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A060ZAA1_ONCMY Publication Abstract from PubMed DNA transposons can be employed for stable gene transfer in vertebrates. The Sleeping Beauty (SB) DNA transposon has been recently adapted for human application and is being evaluated in clinical trials, however its molecular mechanism is not clear. SB transposition is catalyzed by the transposase enzyme, which is a multi-domain protein containing the catalytic and the DNA-binding domains. The DNA-binding domain of the SB transposase contains two structurally independent subdomains, PAI and RED. Recently, the structures of the catalytic domain and the PAI subdomain have been determined, however no structural information on the RED subdomain and its interactions with DNA has been available. Here, we used NMR spectroscopy to determine the solution structure of the RED subdomain and characterize its interactions with the transposon DNA. NMR solution structure of the RED subdomain of the Sleeping Beauty transposase.,Konnova TA, Singer CM, Nesmelova IV Protein Sci. 2017 Jun;26(6):1171-1181. doi: 10.1002/pro.3167. Epub 2017 Apr 2. PMID:28345263 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Transposase 3D structures

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