6ux5 | pdb_00006ux5

Structure of acrorhagin I from the sea anemone Actinia equina

spinqualitylabelsall models 6ux5 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ux5 is a 1 chain structure with sequence from Actinia equina. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ACR1_ACTEQ Toxin. Publication Abstract from PubMed Acrorhagin I (U-AITX-Aeq5a) is a disulfide-rich peptide identified in the aggressive organs (acrorhagi) of the sea anemone Actinia equina. Previous studies (Toxicon 2005, 46:768-74) found that the peptide is toxic in crabs, although the structural and functional properties of acrorhagin I have not been reported. In this work, an Escherichia coli (BL21 strain) expression system was established for the preparation of (13)C,(15)N-labelled acrorhagin I, and the solution structure was determined using NMR spectroscopy. Structurally, acrorhagin I is similar to B-IV toxin from the marine worm Cerebratulus lacteus (PDB id 1VIB), with a well-defined helical hairpin structure stabilised by four intramolecular disulfide bonds. The recombinant peptide was tested in patch-clamp electrophysiology assays against voltage-gated potassium and sodium channels, and in bacterial and fungal growth inhibitory assays and haemolytic assays. Acrorhagin I was not active against any of the ion channels tested and showed no activity in functional assays, indicating that this peptide may possess a different biological function. Metal ion interaction studies using NMR spectroscopy showed that acrorhagin I bound zinc and nickel, suggesting that its function might be modulated by metal ions or that it may be involved in regulating metal ion levels and their transport. The similarity between the structure of acrorhagin I and that of B-IV toxin from a marine worm suggests that this fold may prove to be a recurring motif in disulfide-rich peptides from marine organisms. A disulfide-stabilised helical hairpin fold in acrorhagin I: An emerging structural motif in peptide toxins.,Krishnarjuna B, Sunanda P, Villegas-Moreno J, Csoti A, A V Morales R, Wai DCC, Panyi G, Prentis P, Norton RS J Struct Biol. 2020 Dec 30;213(2):107692. doi: 10.1016/j.jsb.2020.107692. PMID:33387653 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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