6vvj | pdb_00006vvj

Cap1G-TPUA

spinqualitylabelsall models 6vvj Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6vvj is a 1 chain structure with sequence from Human immunodeficiency virus 1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: M7G Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Heterogeneous transcriptional start site usage by HIV-1 produces 5'-capped RNAs beginning with one, two, or three 5'-guanosines ((Cap)1G, (Cap)2G, or (Cap)3G, respectively) that are either selected for packaging as genomes ((Cap)1G) or retained in cells as translatable messenger RNAs (mRNAs) ((Cap)2G and (Cap)3G). To understand how 5'-guanosine number influences fate, we probed the structures of capped HIV-1 leader RNAs by deuterium-edited nuclear magnetic resonance. The (Cap)1G transcript adopts a dimeric multihairpin structure that sequesters the cap, inhibits interactions with eukaryotic translation initiation factor 4E, and resists decapping. The (Cap)2G and (Cap)3G transcripts adopt an alternate structure with an elongated central helix, exposed splice donor residues, and an accessible cap. Extensive remodeling, achieved at the energetic cost of a G-C base pair, explains how a single 5'-guanosine modifies the function of a ~9-kilobase HIV-1 transcript. Structural basis for transcriptional start site control of HIV-1 RNA fate.,Brown JD, Kharytonchyk S, Chaudry I, Iyer AS, Carter H, Becker G, Desai Y, Glang L, Choi SH, Singh K, Lopresti MW, Orellana M, Rodriguez T, Oboh U, Hijji J, Ghinger FG, Stewart K, Francis D, Edwards B, Chen P, Case DA, Telesnitsky A, Summers MF Science. 2020 Apr 24;368(6489):413-417. doi: 10.1126/science.aaz7959. PMID:32327595 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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