6yeg | pdb_00006yeg

Hybrid structure of the SPP1 tail tube by solid-state NMR and cryo EM - Final EM Refinement

spinqualitylabelsall models 6yeg, resolution 4.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6yeg is a 12 chain structure with sequence from Bacillus phage SPP1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TUBE_BPSPP Forms the 160 nm long, sixfold helical, rigid tail tube. Binding to the entry receptor triggers structural rearrangements of the tail tube leading to ejection of the phage DNA into the host. Publication Abstract from PubMed Bacteriophage SPP1 is a double-stranded DNA virus of the Siphoviridae family that infects the bacterium Bacillus subtilis. This family of phages features a long, flexible, non-contractile tail that has been difficult to characterize structurally. Here, we present the atomic structure of the tail tube of phage SPP1. Our hybrid structure is based on the integration of structural restraints from solid-state nuclear magnetic resonance (NMR) and a density map from cryo-EM. We show that the tail tube protein gp17.1 organizes into hexameric rings that are stacked by flexible linker domains and, thus, form a hollow flexible tube with a negatively charged lumen suitable for the transport of DNA. Additionally, we assess the dynamics of the system by combining relaxation measurements with variances in density maps. Architecture of the flexible tail tube of bacteriophage SPP1.,Zinke M, Sachowsky KAA, Oster C, Zinn-Justin S, Ravelli R, Schroder GF, Habeck M, Lange A Nat Commun. 2020 Nov 13;11(1):5759. doi: 10.1038/s41467-020-19611-1. PMID:33188213 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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