7ely | pdb_00007ely

A peptide with high affinity for B-Cell lymphoma2(Bcl-2)

spinqualitylabelsall models 7ely Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7ely is a 1 chain structure with sequence from Escherichia virus M13. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Natural disulfide-rich peptides (DRPs) are valuable scaffolds for the development of new bioactive molecules and therapeutics. However, there are only a limited number of topologically distinct DRP folds in nature, and most of them suffer from the problem of in vitro oxidative folding. Thus, strategies to design DRPs with new constrained topologies beyond the scope of natural folds are desired. Herein we report a general evolution-inspired strategy to design new DRPs with diverse disulfide frameworks, which relies on the incorporation of two cysteine residues and a random peptide sequence into a precursor disulfide-stabilized fold. These peptides can spontaneously fold in redox buffers to the expected tricyclic topologies with high yields. Moreover, we demonstrated that these DRPs can be used as templates for the construction of phage-displayed peptide libraries, enabling the discovery of new DRP ligands from fully randomized sequences. This study thus paves the way for the development of new DRP ligands and therapeutics with structures not derived from natural DRPs. An evolution-inspired strategy to design disulfide-rich peptides tolerant to extensive sequence manipulation.,Zha J, Li J, Fan S, Duan Z, Zhao Y, Wu C Chem Sci. 2021 Jul 22;12(34):11464-11472. doi: 10.1039/d1sc02952e. eCollection, 2021 Sep 1. PMID:34567500 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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