8hf2 | pdb_00008hf2
Cryo-EM structure of WeiTsing
Structural highlights
FunctionPublication Abstract from PubMedPlant roots encounter numerous pathogenic microbes that often cause devastating diseases. One such pathogen, Plasmodiophora brassicae (Pb), causes clubroot disease and severe yield losses on cruciferous crops worldwide. Here, we report the isolation and characterization of WeiTsing (WTS), a broad-spectrum clubroot resistance gene from Arabidopsis. WTS is transcriptionally activated in the pericycle upon Pb infection to prevent pathogen colonization in the stele. Brassica napus carrying the WTS transgene displayed strong resistance to Pb. WTS encodes a small protein localized in the endoplasmic reticulum (ER), and its expression in plants induces immune responses. The cryoelectron microscopy (cryo-EM) structure of WTS revealed a previously unknown pentameric architecture with a central pore. Electrophysiology analyses demonstrated that WTS is a calcium-permeable cation-selective channel. Structure-guided mutagenesis indicated that channel activity is strictly required for triggering defenses. The findings uncover an ion channel analogous to resistosomes that triggers immune signaling in the pericycle. WeiTsing, a pericycle-expressed ion channel, safeguards the stele to confer clubroot resistance.,Wang W, Qin L, Zhang W, Tang L, Zhang C, Dong X, Miao P, Shen M, Du H, Cheng H, Wang K, Zhang X, Su M, Lu H, Li C, Gao Q, Zhang X, Huang Y, Liang C, Zhou JM, Chen YH Cell. 2023 Jun 8;186(12):2656-2671.e18. doi: 10.1016/j.cell.2023.05.023. Epub , 2023 Jun 8. PMID:37295403[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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