5d6j | pdb_00005d6j

Crystal structure of a mycobacterial protein

spinqualitylabelsall models 5d6j, resolution 2.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5d6j is a 2 chain structure with sequence from Mycolicibacterium smegmatis MC2 155 and Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ATP, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SMT3_YEAST Not known; suppressor of MIF2 mutations. Publication Abstract from PubMed Fatty acid degradation protein D32 (FadD32), an enzyme required for mycolic acid biosynthesis and essential for mycobacterial growth, has recently been identified as a valid and promising target for anti-tuberculosis drug development. Here we report the crystal structures of Mycobacterium smegmatis FadD32 in the apo and ATP-bound states at 2.4 A and 2.25 A resolution, respectively. FadD32 consists of two globular domains connected by a flexible linker. ATP binds in a cleft at the interface between the N- and C-terminal domains and its binding induces significant local conformational changes in FadD32. The binding sites of meromycolic acid and phosphopantetheine are identified by structural comparison with other members of the adenylating enzyme superfamily. These results will improve our understanding of the catalytic mechanism of FadD32 and help in the design of inhibitors of this essential enzyme. Crystal structure of FadD32, an enzyme essential for mycolic acid biosynthesis in mycobacteria.,Li W, Gu S, Fleming J, Bi L Sci Rep. 2015 Dec 2;5:15493. doi: 10.1038/srep15493. PMID:26628098 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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