8ciw | pdb_00008ciw

Methylsuccinyl-CoA dehydrogenase from Pseudomonas migulae with bound FAD and (2S)-methylsuccinyl-CoA

spinqualitylabelsall models 8ciw, resolution 1.93Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8ciw is a 2 chain structure with sequence from Pseudomonas migulae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.93Å Ligands: COA, FAD, FLC, P6G, ZKK Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1H5ILA2_9PSED Publication Abstract from PubMed Nature has evolved eight different pathways for the capture and conversion of CO(2), including the Calvin-Benson-Bassham cycle of photosynthesis. Yet, these pathways underlie constrains and only represent a fraction of the thousands of theoretically possible solutions. To overcome the limitations of natural evolution, we introduce the HydrOxyPropionyl-CoA/Acrylyl-CoA (HOPAC) cycle, a new-to-nature CO(2)-fixation pathway that was designed through metabolic retrosynthesis around the reductive carboxylation of acrylyl-CoA, a highly efficient principle of CO(2) fixation. We realized the HOPAC cycle in a step-wise fashion and used rational engineering approaches and machine learning-guided workflows to further optimize its output by more than one order of magnitude. Version 4.0 of the HOPAC cycle encompasses 11 enzymes from six different organisms, converting ~3.0 mM CO(2) into glycolate within 2 hours. Our work moves the hypothetical HOPAC cycle from a theoretical design into an established in vitro system that forms the basis for different potential applications. Exploring alternative pathways for the in vitro establishment of the HOPAC cycle for synthetic CO(2) fixation.,McLean R, Schwander T, Diehl C, Cortina NS, Paczia N, Zarzycki J, Erb TJ Sci Adv. 2023 Jun 16;9(24):eadh4299. doi: 10.1126/sciadv.adh4299. Epub 2023 Jun , 14. PMID:37315145 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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