5fag | pdb_00005fag

Alanine Racemase from Streptomyces coelicolor A3(2) with Bound Propionate Inhibitor

spinqualitylabelsall models 5fag, resolution 1.51Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5fag is a 4 chain structure with sequence from Streptomyces coelicolor A3(2). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.51Å Ligands: KCX, NA, NO3, PLP, PPI Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ALR_STRCO Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids. Publication Abstract from PubMed The conversion of l-alanine (L-Ala) into d-alanine (D-Ala) in bacteria is performed by pyridoxal phosphate-dependent enzymes called alanine racemases. D-Ala is an essential component of the bacterial peptidoglycan and hence required for survival. The Gram-positive bacterium Streptomyces coelicolor has at least one alanine racemase encoded by alr. Here, we describe an alr deletion mutant of S. coelicolor which depends on D-Ala for growth and shows increased sensitivity to the antibiotic d-cycloserine (DCS). The crystal structure of the alanine racemase (Alr) was solved with and without the inhibitors DCS or propionate, at 1.64 A and 1.51 A resolution, respectively. The crystal structures revealed that Alr is a homodimer with residues from both monomers contributing to the active site. The dimeric state of the enzyme in solution was confirmed by gel filtration chromatography, with and without L-Ala or d-cycloserine. The activity of the enzyme was 66 +/- 3 U mg-1 for the racemization of L- to D-Ala, and 104 +/- 7 U mg-1 for the opposite direction. Comparison of Alr from S. coelicolor with orthologous enzymes from other bacteria, including the closely related d-cycloserine-resistant Alr from S. lavendulae, strongly suggests that structural features such as the hinge angle or the surface area between the monomers do not contribute to d-cycloserine resistance, and the molecular basis for resistance therefore remains elusive. Structural and functional characterization of the alanine racemase from Streptomyces coelicolor A3(2).,Tassoni R, van der Aart LT, Ubbink M, van Wezel GP, Pannu NS Biochem Biophys Res Commun. 2017 Jan 29;483(1):122-128. doi:, 10.1016/j.bbrc.2016.12.183. Epub 2016 Dec 29. PMID:28042035 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Alanine racemase 3D structures

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