5gj9 | pdb_00005gj9
Crystal structure of Arabidopsis thaliana ACO2 in complex with POA
Structural highlights
FunctionACCO2_ARATH Enzyme involved in the ethylene biosynthesis. Required to mediate the 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated reversion of the ABA-induced inhibition of seed germination via endosperm rupture. May promote stem elongation by maximizing the extensibility cells, possibly by activating ethylene biosynthesis, in response to very-long-chain fatty acids (VLCFAs C20:0 to C30:0).[1] [2] Publication Abstract from PubMedEthylene is an important phytohormone that promotes the ripening of fruits and senescence of flowers thereby reducing their shelf lives. Specific ethylene biosynthesis inhibitors would help to decrease postharvest loss. Here, we identify pyrazinamide (PZA), a clinical drug used to treat tuberculosis, as an inhibitor of ethylene biosynthesis in Arabidopsis thaliana, using a chemical genetics approach. PZA is converted to pyrazinecarboxylic acid (POA) in plant cells, suppressing the activity of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), the enzyme catalysing the final step of ethylene formation. The crystal structures of Arabidopsis ACO2 in complex with POA or 2-Picolinic Acid (2-PA), a POA-related compound, reveal that POA/2-PA bind at the active site of ACO, preventing the enzyme from interacting with its natural substrates. Our work suggests that PZA and its derivatives may be promising regulators of plant metabolism, in particular ethylene biosynthesis. Pyrazinamide and derivatives block ethylene biosynthesis by inhibiting ACC oxidase.,Sun X, Li Y, He W, Ji C, Xia P, Wang Y, Du S, Li H, Raikhel N, Xiao J, Guo H Nat Commun. 2017 Jun 12;8:15758. doi: 10.1038/ncomms15758. PMID:28604689[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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