5goa | pdb_00005goa

Cryo-EM structure of RyR2 in open state

Loading... Status: waiting for Mol* initialization Structural highlights 5goa is a 4 chain structure with sequence from Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.2Å Ligands: ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed RyR2 is a high-conductance intracellular calcium (Ca2+) channel that controls the release of Ca2+ from the sarco(endo)plasmic reticulum of a variety of cells. Here, we report the structures of RyR2 from porcine heart in both the open and closed states at near-atomic resolutions determined using single-particle electron cryomicroscopy. Structural comparison reveals a breathing motion of the overall cytoplasmic region resulted from the interdomain movements of amino-terminal domains (NTDs), Helical domains, and Handle domains, whereas almost no intradomain shifts are observed in these armadillo repeats-containing domains. Outward rotations of the Central domains, which integrate the conformational changes of the cytoplasmic region, lead to the dilation of the cytoplasmic gate through coupled motions. Our structural and mutational characterizations provide important insights into the gating and disease mechanism of RyRs. Structural basis for the gating mechanism of the type 2 ryanodine receptor RyR2.,Peng W, Shen H, Wu J, Guo W, Pan X, Wang R, Chen SR, Yan N Science. 2016 Oct 21;354(6310). pii: aah5324. Epub 2016 Sep 22. PMID:27708056 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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