5gwp | pdb_00005gwp

Crystal structure of RCAR3:PP2C wild-type with (+)-ABA

spinqualitylabelsall models 5gwp, resolution 2.58Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5gwp is a 4 chain structure with sequence from Oryza sativa and Oryza sativa Japonica Group. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.577Å Ligands: A8S, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PYL3_ORYSJ Involved in abscisic acid (ABA) signaling during seed germination and abiotic stress response. Acts as positive regulator of ABA-mediated inhibition of seed germination, and tolerance to drought and cold stresses (PubMed:26362328). Together with PP2C50 and SAPK10, may form an ABA signaling module involved in stress response (PubMed:28827170). Inhibits the protein phosphatases PP2C06 and PP2C09 when activated by abscisic acid (ABA) (PubMed:24743650). Publication Abstract from PubMed Abscisic acid (ABA) signaling pathway is regulated by Clade A type 2C protein phosphatases (PP2CAs) in plant. In the presence of ABA, PP2Cs release stress/ABA-activated protein kinases by binding to ABA-bound receptors (PYL/RCARs) for activation. Although the wedging tryptophan in PP2Cs is critical in the interaction with PYL/RCARs in Arabidopsis and rice, it remains elusive how other interface regions are involved in the interaction. Here, we identified a conserved region on PP2Cs, termed VxGPhiL motif, that modulates the interaction with PYL/RCARs through its second and forth residues. The effects of the second and forth residues on the interaction of OsPP2C50 with several OsPYL/RCAR proteins were investigated by systematic mutagenesis. Investigating the interaction between OsPP2C50 and OsPYL/RCAR3, one OsPP2C50 mutant, VFGML ("FM") mutant, lowered the affinity by approximately 15-fold. Comparison of the crystal structures of OsPP2C50:ABA:OsPYL/RCAR3 wild-type and FM mutant revealed local conformational changes near the VxGPhiL motif, further supported by hydrogen-deuterium exchange mass spectrometry. In rice protoplasts, ABA signaling was altered by mutations in the VxGPhiL motif. Transgenic Arabidopsis plants overexpressing OsPP2C50 and OsPP2C50FM changed ABA sensitivity, validating the in vitro results. Taken together, the VxGPhiL motif of PP2Cs seems to modulate the affinity to PYL/RCARs with potentials to alter the ABA signaling, leading to the differential sensitivity to ABA in planta. Modulation of ABA signaling by altering VxGPhiL motif of PP2Cs in Oryza sativa.,Han S, Min MK, Lee SY, Lim CW, Bhatnagar N, Lee Y, Shin D, Chung KY, Lee SC, Kim BG, Lee S Mol Plant. 2017 Aug 18. pii: S1674-2052(17)30233-2. doi:, 10.1016/j.molp.2017.08.003. PMID:28827170 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA