1ybk | pdb_00001ybk

RHCC cocrystallized with CAPB

spinqualitylabelsall models 1ybk, resolution 1.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1ybk is a 4 chain structure with sequence from Staphylothermus marinus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.45Å Ligands: BEQ, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q54436_STAMA Publication Abstract from PubMed Crystals of excellent quality are a prerequisite for high-resolution X-ray data. However, in refinement protocols of crystallization conditions it is often difficult to obtain the right combination of, for example, protein concentration, drop size, temperature and additives. A novel approach for optimizing crystal contacts in a most favourable fashion by performing crystallization setups with the zwitterionic surfactant cocoamidoproylbetaine (CAPB) is introduced. In the presence of this surfactant, highly diffracting crystals were obtained. Here, data from a right-handed coiled coil (RHCC) in complex with CAPB at 1.4 A resolution are presented. The addition of CAPB using otherwise identical crystallization conditions and the same X-ray source caused an improvement in resolution from 2.9 to 1.4 A. Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB).,Ozbek S, Muller JF, Figgemeier E, Stetefeld J Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):477-80. Epub 2005, Mar 24. PMID:15805603 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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