5hww | pdb_00005hww
Crystal structure of PAS1 complexed with 1,2,4-TMB
Structural highlights
FunctionTODS_PSEP1 Member of the two-component regulatory system TodS/TodT involved in the regulation of toluene degradation. Phosphorylates TodT via a four-step phosphorelay in response to toluene (Probable).[1] Publication Abstract from PubMedTodS is a sensor kinase that responds to various monoaromatic compounds, which either cause an agonistic or antagonistic effect on phosphorylation of its cognate response regulator TodT, and controls tod operon expression in Pseudomonas putida strains. We describe a molecular sensing mechanism of TodS that is activated in response to toluene. The crystal structures of the TodS Per-Arnt-Sim (PAS) 1 sensor domain (residues 43-164) and its complex with toluene (agonist) or 1,2,4-trimethylbenzene (antagonist) show a typical beta2alpha3beta3 PAS fold structure (residues 45-149), forming a hydrophobic ligand-binding site. A signal transfer region (residues 150-163) located immediately after the canonical PAS fold may be intrinsically flexible and disordered in both apo-PAS1 and antagonist-bound forms and dramatically adapt an alpha-helix upon toluene binding. This structural change in the signal transfer region is proposed to result in signal transmission to activate the TodS/TodT two-component signal transduction system. Site-directed mutagenesis and beta-galactosidase assays using a P. putida reporter strain system verified the essential residues involved in ligand sensing and signal transfer and suggest that the Phe(46) residue acts as a ligand-specific switch. Molecular Insights into Toluene Sensing in the TodS/TodT Signal Transduction System.,Koh S, Hwang J, Guchhait K, Lee EG, Kim SY, Kim S, Lee S, Chung JM, Jung HS, Lee SJ, Ryu CM, Lee SG, Oh TK, Kwon O, Kim MH J Biol Chem. 2016 Apr 15;291(16):8575-90. doi: 10.1074/jbc.M116.718841. Epub 2016, Feb 22. PMID:26903514[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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