1m5o | pdb_00001m5o
Transition State Stabilization by a Catalytic RNA
|
Overview
The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through, transesterification of the scissile phosphate. Vanadate has previously, been used as a transition state mimic of protein enzymes that catalyze the, same reaction. Comparison of the 2.2 angstrom resolution structure of a, vanadate-hairpin ribozyme complex with structures of precursor and product, complexes reveals a rigid active site that makes more hydrogen bonds to, the transition state than to the precursor or product. Because of the, paucity of RNA functional groups capable of general acid-base or, electrostatic catalysis, transition state stabilization is likely to be an, important catalytic strategy for ribozymes.
About this Structure
1M5O is a Single protein structure of sequence from Homo sapiens with CA as ligand. Full crystallographic information is available from OCA.
Reference
Transition state stabilization by a catalytic RNA., Rupert PB, Massey AP, Sigurdsson ST, Ferre-D'Amare AR, Science. 2002 Nov 15;298(5597):1421-4. Epub 2002 Oct 10. PMID:12376595
Page seeded by OCA on Mon Nov 12 18:07:45 2007
