2gim | pdb_00002gim

1.6 Angstrom structure of plastocyanin from Anabaena variabilis

spinqualitylabelsall models 2gim, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2gim is a 2 chain structure with sequence from Trichormus variabilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.6Å Ligands: CU Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PLAS_ANAVA Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in Escherichia coli and purified. Plate-like crystals were obtained by crystallization in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized protein was solved to a resolution of 1.6 Angstrom using plastocyanin from Phormidium laminosum as a search model. Two molecules were found in the asymmetric unit. The electrostatic surface of the basic protein showed a large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged patches. The copper ion was found to be relatively mobile and there were two distinct conformations of His61. Structure of plastocyanin from the cyanobacterium Anabaena variabilis.,Schmidt L, Christensen HE, Harris P Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1022-9. Epub 2006, Aug 19. PMID:16929103 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Plastocyanin 3D structures

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