3i17 | pdb_00003i17

Crystal structure of the apo R132K:L121E mutant of cellular retinoic acid-binding protein II at 1.68 angstrom resolution

spinqualitylabelsall models 3i17, resolution 1.68Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3i17 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.68Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RABP2_HUMAN Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Cellular Retinoic Acid Binding Protein II (CRABPII) has been reengineered to specifically bind and react with all-trans-retinal to form a protonated Schiff base. Each step of this process has been dissected and four residues (Lys132, Tyr134, Arg111, and Glu121) within the CRABPII binding site have been identified as crucial for imine formation and/or protonation. The precise role of each residue has been examined through site directed mutagenesis and crystallographic studies. The crystal structure of the R132K:L121E-CRABPII (PDB-3I17) double mutant suggests a direct interaction between engineered Glu121 and the native Arg111, which is critical for both Schiff base formation and protonation. Proteins 2009. (c) 2009 Wiley-Liss, Inc. Elucidating the exact role of engineered CRABPII residues for the formation of a retinal protonated Schiff base.,Vasileiou C, Wang W, Jia X, Lee KS, Watson CT, Geiger JH, Borhan B Proteins. 2009 Jun 8. PMID:19603486 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also CRABP I ( Cellular Retinoic Acid Binding Protein ) Cellular retinoic acid-binding protein 3D structures Gustavo Elberto Epalza Sanchez/Sandbox 1 Molecular Playground/CRABP I (Cellular Retinoic Acid Binding Protein)

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