5j4z | pdb_00005j4z

Architecture of tight respirasome

Loading... Status: waiting for Mol* initialization Structural highlights 5j4z is a 10 chain structure with sequence from Ovis aries. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 5.8Å Ligands: CU, CUA, FES, HEA, HEM, SF4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Mitochondrial electron transport chain complexes are organized into supercomplexes responsible for carrying out cellular respiration. Here we present three architectures of mammalian (ovine) supercomplexes determined by cryo-electron microscopy. We identify two distinct arrangements of supercomplex CICIII2CIV (the respirasome)-a major 'tight' form and a minor 'loose' form (resolved at the resolution of 5.8 A and 6.7 A, respectively), which may represent different stages in supercomplex assembly or disassembly. We have also determined an architecture of supercomplex CICIII2 at 7.8 A resolution. All observed density can be attributed to the known 80 subunits of the individual complexes, including 132 transmembrane helices. The individual complexes form tight interactions that vary between the architectures, with complex IV subunit COX7a switching contact from complex III to complex I. The arrangement of active sites within the supercomplex may help control reactive oxygen species production. To our knowledge, these are the first complete architectures of the dominant, physiologically relevant state of the electron transport chain. The architecture of respiratory supercomplexes.,Letts JA, Fiedorczuk K, Sazanov LA Nature. 2016 Sep 21;537(7622):644-648. doi: 10.1038/nature19774. PMID:27654913 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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