8okv | pdb_00008okv

lipoprotein BT2095 from Bacteroides thetaiotamicron bound to cyanocobalamin CnCbl

spinqualitylabelsall models 8okv, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8okv is a 4 chain structure with sequence from Bacteroides thetaiotaomicron VPI-5482. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.6Å Ligands: CNC Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8A5Z1_BACTN Publication Abstract from PubMed Vitamin B(12) (cobalamin) is required for most human gut microbes, many of which are dependent on scavenging to obtain this vitamin. Since bacterial densities in the gut are extremely high, competition for this keystone micronutrient is severe. Contrasting with Enterobacteria, members of the dominant genus Bacteroides often encode several BtuB vitamin B(12) outer membrane transporters together with a conserved array of surface-exposed B(12)-binding lipoproteins. Here we show that the BtuB transporters from Bacteroides thetaiotaomicron form stable, pedal bin-like complexes with surface-exposed BtuG lipoprotein lids, which bind B(12) with high affinities. Closing of the BtuG lid following B(12) capture causes destabilisation of the bound B(12) by a conserved BtuB extracellular loop, causing translocation of the vitamin to BtuB and subsequent transport. We propose that TonB-dependent, lipoprotein-assisted small molecule uptake is a general feature of Bacteroides spp. that is important for the success of this genus in colonising the human gut. BtuB TonB-dependent transporters and BtuG surface lipoproteins form stable complexes for vitamin B(12) uptake in gut Bacteroides.,Abellon-Ruiz J, Jana K, Silale A, Frey AM, Basle A, Trost M, Kleinekathofer U, van den Berg B Nat Commun. 2023 Aug 5;14(1):4714. doi: 10.1038/s41467-023-40427-2. PMID:37543597 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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