4pz5 | pdb_00004pz5

Crystal structure of the second and third fibronectin F1 modules in complex with a fragment of BBK32 from Borrelia burgdorferi

spinqualitylabelsall models 4pz5, resolution 1.96Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4pz5 is a 2 chain structure with sequence from Borreliella burgdorferi B31 and Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.96Å Ligands: ACE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function O50835_BORBU Publication Abstract from PubMed BBK32 is a fibronectin (FN)[minus]binding protein expressed on the cell surface of Borrelia burgdorferi, the causative agent of Lyme disease. There is conflicting information about where and how BBK32 interacts with FN. We have characterized interactions of a recombinant 86[minus]mer polypeptide, Bbk32, comprising the unstructured FN[minus]binding region of BBK32. Competitive enzyme[minus]linked assays utilizing various FN fragments and epitope[minus]mapped anti[minus]FN monoclonal antibodies showed that Bbk32 binding involves both the fibrin[minus] and gelatin[minus]binding domains of the 70 kDa N[minus]terminal region (FN70K). Crystallographic and NMR analyses of smaller Bbk32 peptides complexed, respectively, with 2[minus]3FNI and 8[minus]9FNI, demonstrated that binding occurs by beta[minus]strand addition. Isothermal titration calorimetry indicated that Bbk32 binds to isolated FN70K more tightly than to intact FN. In a competitive enzyme[minus]linked binding assay, complex formation with Bbk32 enhanced binding of FN with mAbIII[minus]10 to the 10FNIII module. Thus, Bbk32 binds to multiple FN type 1 modules of the FN70K region by a tandem beta[minus]zipper mechanism, and in doing so increases accessibility of FNIII modules that interact with other ligands. The similarity in the FN[minus]binding mechanism of BBK32 and previously studied streptococcal proteins suggests that the binding and associated conformational change of FN play a role in infection. Borrelia burgdorferi protein BBK32 binds to soluble fibronectin via the N-terminal 70 kDa region, causing fibronectin to undergo conformational extension.,Harris G, Ma W, Maurer LM, Potts JR, Mosher DF J Biol Chem. 2014 Jun 24. pii: jbc.M114.578419. PMID:24962582 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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