4y6w | pdb_00004y6w

Crystal structure of Podosopora anserina putative kinesin light chain nearly identical TPR-like repeats

spinqualitylabelsall models 4y6w, resolution 1.59Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4y6w is a 1 chain structure with sequence from Podospora anserina. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.587Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A090CRQ5_PODAN Publication Abstract from PubMed Linear repeat proteins often have high structural similarity and low ( approximately 25%) pairwise sequence identities (PSI) among modules. We identified a unique P. anserina (Pa) sequence with tetratricopeptide repeat (TPR) homology, which contains longer (42 residue) repeats (42PRs) with an average PSI >91%. We determined the crystal structure of five tandem Pa 42PRs to 1.6 A, and examined the stability and solution properties of constructs containing three to six Pa 42PRs. Compared with 34-residue TPRs (34PRs), Pa 42PRs have a one-turn extension of each helix, and bury more surface area. Unfolding transitions shift to higher denaturant concentration and become sharper as repeats are added. Fitted Ising models show Pa 42PRs to be more cooperative than consensus 34PRs, with increased magnitudes of intrinsic and interfacial free energies. These results demonstrate the tolerance of the TPR motif to length variation, and provide a basis to understand the effects of helix length on intrinsic/interfacial stability. A Naturally Occurring Repeat Protein with High Internal Sequence Identity Defines a New Class of TPR-like Proteins.,Marold JD, Kavran JM, Bowman GD, Barrick D Structure. 2015 Oct 1. pii: S0969-2126(15)00369-X. doi:, 10.1016/j.str.2015.07.022. PMID:26439765 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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