5kle | pdb_00005kle

Structure of CBM_E1, a novel carbohydrate-binding module found by sugar cane soil metagenome, complexed with cellopentaose

spinqualitylabelsall models 5kle, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5kle is a 1 chain structure with sequence from Uncultured bacterium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.5Å Ligands: BGC, PRD_900016 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0R5P8X1_9BACT Publication Abstract from PubMed Carbohydrate-binding modules (CBMs) are appended to glycoside hydrolases and can contribute to the degradation of complex recalcitrant substrates such as the plant cell wall. For application in bioethanol production, novel enzymes with high catalytic activity against recalcitrant lignocellulosic material are being explored and developed. In this work, we report the functional and structural study of CBM_E1, discovered through a metagenomics approach, which is the founding member of a novel CBM family, CBMxx. CBM_E1, which is linked to an endoglucanase, displayed affinity for mixed linked beta-1,4-beta-1,3-glucans, xyloglucan, avicel and cellooligosaccharides. The crystal structure of CBM_E1 in complex with cellopentaose displayed a canonical beta-sandwich fold comprising two beta sheets. The planar ligand binding site, observed in a parallel orientation with the beta strands, is a typical feature of Type A CBMs. On the other hand, affinity for bacterial crystalline cellulose was not detected, a ligand recognized by Type A CBMs, while binding to soluble glucans was enthalpically driven, typical of Type B modules. These unique properties of CBM_E1 are at the interface between Type A and Type B CBMs. A novel carbohydrate-binding module from sugar cane soil metagenome featuring unique structural and carbohydrate affinity properties.,Campos BM, Liberato MV, Alvarez TM, Zanphorlin LM, Ematsu GC, Barud H, Polikarpov I, Ruller R, Gilbert HJ, Zeri AC, Squina FM J Biol Chem. 2016 Sep 12. pii: jbc.M116.744383. PMID:27621314 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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