8ca9 | pdb_00008ca9

Cryo-EM structure of the Cibeles-Demetra 3:3 heterocomplex from Galleria mellonella saliva

spinqualitylabelsall models 8ca9, resolution 2.29Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8ca9 is a 6 chain structure with sequence from Galleria mellonella. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.29Å Ligands: BMA, CU, MAN, NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ARY_GALME Larval storage protein (LSP) which may serve as a store of amino acids for synthesis of adult proteins (Probable). Binds the A.niger cell wall component alpha-1,3-glucan, a fungal pathogen-associated molecular pattern (PAMP) that activates the host immune response (PubMed:34443685). Publication Abstract from PubMed Plastic waste management is a pressing ecological, social, and economic challenge. The saliva of the lepidopteran Galleria mellonella larvae is capable of oxidizing and depolymerizing polyethylene in hours at room temperature. Here, we analyze by cryo-electron microscopy (cryo-EM) G. mellonella's saliva directly from the native source. The three-dimensional reconstructions reveal that the buccal secretion is mainly composed of four hexamerins belonging to the hemocyanin/phenoloxidase family, renamed Demetra, Cibeles, Ceres, and a previously unidentified factor termed Cora. Functional assays show that this factor, as its counterparts Demetra and Ceres, is also able to oxidize and degrade polyethylene. The cryo-EM data and the x-ray analysis from purified fractions show that they self-assemble primarily into three macromolecular complexes with striking structural differences that likely modulate their activity. Overall, these results establish the ground to further explore the hexamerins' functionalities, their role in vivo, and their eventual biotechnological application. Plastic degradation by insect hexamerins: Near-atomic resolution structures of the polyethylene-degrading proteins from the wax worm saliva.,Spinola-Amilibia M, Illanes-Vicioso R, Ruiz-L Formula See Text Pez E, Colomer-Vidal P, Rodriguez FV, Peces Perez R, Arias CF, Torroba T, Sol Formula See Text M, Arias-Palomo E, Bertocchini F Sci Adv. 2023 Sep 22;9(38):eadi6813. doi: 10.1126/sciadv.adi6813. Epub 2023 Sep , 20. PMID:37729416 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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