8h8m | pdb_00008h8m
Crystal structure of apo-E53F/E57F/E60F/E64F-rHLFr
Structural highlights
FunctionFRIL_HORSE Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Publication Abstract from PubMedMultiple aromatic residues assemble to form higher ordered structures known as "aromatic clusters" in proteins and play essential roles in biological systems. However, the stabilization mechanism and dynamic behavior of aromatic clusters remain unclear. This study describes designed aromatic interactions confined within a protein cage to reveal how aromatic clusters affect protein stability. The crystal structures and calorimetric measurements indicate that the formation of inter-subunit phenylalanine clusters enhance the interhelix interactions and increase the melting temperature. Theoretical calculations suggest that this is caused by the transformation of the T-shaped geometry into pi-pi stacking at high temperatures, and the hydration entropic gain. Thus, the isolated nanoenvironment in a protein cage allows reconstruction and detailed analysis of multiple clustering residues for elucidating the mechanisms of various biomolecular interactions in nature which can be applied to design of bionanomaterials. Elucidating Conformational Dynamics and Thermostability of Designed Aromatic Clusters by Using Protein Cages.,Hishikawa Y, Noya H, Nagatoishi S, Yoshidome T, Maity B, Tsumoto K, Abe S, Ueno T Chemistry. 2023 Jun 19;29(34):e202300488. doi: 10.1002/chem.202300488. Epub 2023 , May 2. PMID:37070368[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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