5wph | pdb_00005wph

Crystal structure of ArsN, N-acetyltransferase with substrate AST from Pseudomonas putida KT2440

spinqualitylabelsall models 5wph, resolution 2.19Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5wph is a 6 chain structure with sequence from Pseudomonas putida KT2440. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.19Å Ligands: BLJ, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q88LK7_PSEPK Publication Abstract from PubMed The emergence and spread of antimicrobial resistance highlights the urgent need for new antibiotics. Organoarsenicals have been used as antimicrobials since Paul Ehrlich's salvarsan. Recently a soil bacterium was shown to produce the organoarsenical arsinothricin. We demonstrate that arsinothricin, a non-proteinogenic analog of glutamate that inhibits glutamine synthetase, is an effective broad-spectrum antibiotic against both Gram-positive and Gram-negative bacteria, suggesting that bacteria have evolved the ability to utilize the pervasive environmental toxic metalloid arsenic to produce a potent antimicrobial. With every new antibiotic, resistance inevitably arises. The arsN1 gene, widely distributed in bacterial arsenic resistance (ars) operons, selectively confers resistance to arsinothricin by acetylation of the alpha-amino group. Crystal structures of ArsN1 N-acetyltransferase, with or without arsinothricin, shed light on the mechanism of its substrate selectivity. These findings have the potential for development of a new class of organoarsenical antimicrobials and ArsN1 inhibitors. Arsinothricin, an arsenic-containing non-proteinogenic amino acid analog of glutamate, is a broad-spectrum antibiotic.,Nadar VS, Chen J, Dheeman DS, Galvan AE, Yoshinaga-Sakurai K, Kandavelu P, Sankaran B, Kuramata M, Ishikawa S, Rosen BP, Yoshinaga M Commun Biol. 2019 Apr 15;2:131. doi: 10.1038/s42003-019-0365-y. eCollection 2019. PMID:30993215 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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