6mjh | pdb_00006mjh

The S31N mutant of the influenza A M2 proton channel in two distinct conformational states

spinqualitylabelsall models 6mjh, resolution 2.06Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6mjh is a 8 chain structure with sequence from Influenza A virus (A/pigeon/Jiangsu/K23/2013(H9N2)). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.06Å Ligands: ACE, CA, CL, NH2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0R5TVW3_9INFA Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry.[RuleBase:RU361247] Publication Abstract from PubMed The amantadine-resistant S31N mutant of the influenza A M2 proton channel has become prevalent in currently circulating viruses. Here we have solved an X-ray crystal structure of M2(22-46) S31N that contains two distinct conformational states within its asymmetric unit. This structure reveals the mechanism of adamantane resistance in both conformational states of the M2 channel. In the Inward(open) conformation, the mutant Asn31 side chain faces the channel pore and sterically blocks the adamantane binding site. In the Inward(closed) conformation, Asn31 forms hydrogen bonds with carbonyls at the monomer-monomer interface, which twists the monomer helices and constricts the channel pore at the drug binding site. We also examine M2(19-49) WT and S31N using solution NMR spectroscopy, and show that distribution of the two conformational states is dependent on both detergent choice and experimental pH. X-ray crystal structure of the influenza A M2 proton channel S31N mutant in two conformational states: an open and shut case.,Thomaston JL, Wu Y, Polizzi N, Liu L, Wang J, DeGrado WF J Am Chem Soc. 2019 Jun 11. doi: 10.1021/jacs.9b02196. PMID:31184871 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ion channels 3D structures

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