6mrv | pdb_00006mrv

Sialidase26 co-crystallized with DANA

spinqualitylabelsall models 6mrv, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6mrv is a 2 chain structure with sequence from Bacterium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: DAN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A4S2B4D9_9BACE Publication Abstract from PubMed Dietary habits have been associated with alterations of the human gut resident microorganisms contributing to obesity, diabetes and cancer(1). In Western diets, red meat is a frequently eaten food(2), but long-term consumption has been associated with increased risk of disease(3,4). Red meat is enriched in N-glycolylneuraminic acid (Neu5Gc) that cannot be synthesized by humans(5). However, consumption can cause Neu5Gc incorporation into cell surface glycans(6), especially in carcinomas(4,7). As a consequence, an inflammatory response is triggered when Neu5Gc-containing glycans encounter circulating anti-Neu5Gc antibodies(8,9). Although bacteria can use free sialic acids as a nutrient source(10-12), it is currently unknown if gut microorganisms contribute to releasing Neu5Gc from food. We found that a Neu5Gc-rich diet induces changes in the gut microbiota, with Bacteroidales and Clostridiales responding the most. Genome assembling of mouse and human shotgun metagenomic sequencing identified bacterial sialidases with previously unobserved substrate preference for Neu5Gc-containing glycans. X-ray crystallography revealed key amino acids potentially contributing to substrate preference. Additionally, we verified that mouse and human sialidases were able to release Neu5Gc from red meat. The release of Neu5Gc from red meat using bacterial sialidases could reduce the risk of inflammatory diseases associated with red meat consumption, including colorectal cancer(4) and atherosclerosis(13). Gut bacteria responding to dietary change encode sialidases that exhibit preference for red meat-associated carbohydrates.,Zaramela LS, Martino C, Alisson-Silva F, Rees SD, Diaz SL, Chuzel L, Ganatra MB, Taron CH, Secrest P, Zuniga C, Huang J, Siegel D, Chang G, Varki A, Zengler K Nat Microbiol. 2019 Sep 23. pii: 10.1038/s41564-019-0564-9. doi:, 10.1038/s41564-019-0564-9. PMID:31548686 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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