6nc6 | pdb_00006nc6

Lipid II flippase MurJ, inward closed conformation

spinqualitylabelsall models 6nc6, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6nc6 is a 2 chain structure with sequence from Thermosipho africanus TCF52B. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.2Å Ligands: CL, NA, OLB, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function MURJ_THEAB Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.[HAMAP-Rule:MF_02078] Publication Abstract from PubMed The biosynthesis of many polysaccharides, including bacterial peptidoglycan and eukaryotic N-linked glycans, requires transport of lipid-linked oligosaccharide (LLO) precursors across the membrane by specialized flippases. MurJ is the flippase for the lipid-linked peptidoglycan precursor Lipid II, a key player in bacterial cell wall synthesis, and a target of recently discovered antibacterials. However, the flipping mechanism of LLOs including Lipid II remains poorly understood due to a dearth of structural information. Here we report crystal structures of MurJ captured in inward-closed, inward-open, inward-occluded and outward-facing conformations. Together with mutagenesis studies, we elucidate the conformational transitions in MurJ that mediate lipid flipping, identify the key ion for function, and provide a framework for the development of inhibitors. Visualizing conformation transitions of the Lipid II flippase MurJ.,Kuk ACY, Hao A, Guan Z, Lee SY Nat Commun. 2019 Apr 15;10(1):1736. doi: 10.1038/s41467-019-09658-0. PMID:30988294 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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