6nmp | pdb_00006nmp

SFX structure of oxidized cytochrome c oxidase at room temperature

spinqualitylabelsall models 6nmp, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6nmp is a 20 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.9Å Ligands: CDL, CHD, CU, CUA, DMU, FME, HEA, MG, NA, PEK, PER, PGV, PSC, SAC, TGL, TPO, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function COX3_BOVIN Subunits I, II and III form the functional core of the enzyme complex. Publication Abstract from PubMed Cytochrome c oxidase (CcO) reduces dioxygen to water and harnesses the chemical energy to drive proton translocation across the inner mitochondrial membrane by an unresolved mechanism. By using time-resolved serial femtosecond crystallography, we identified a key oxygen intermediate of bovine CcO. It is assigned to the PR-intermediate, which is characterized by specific redox states of the metal centers and a distinct protein conformation. The heme a 3 iron atom is in a ferryl (Fe(4+) = O(2-)) configuration, and heme a and CuB are oxidized while CuA is reduced. A Helix-X segment is poised in an open conformational state; the heme a farnesyl sidechain is H-bonded to S382, and loop-I-II adopts a distinct structure. These data offer insights into the mechanism by which the oxygen chemistry is coupled to unidirectional proton translocation. Snapshot of an oxygen intermediate in the catalytic reaction of cytochrome c oxidase.,Ishigami I, Lewis-Ballester A, Echelmeier A, Brehm G, Zatsepin NA, Grant TD, Coe JD, Lisova S, Nelson G, Zhang S, Dobson ZF, Boutet S, Sierra RG, Batyuk A, Fromme P, Fromme R, Spence JCH, Ros A, Yeh SR, Rousseau DL Proc Natl Acad Sci U S A. 2019 Feb 26;116(9):3572-3577. doi:, 10.1073/pnas.1814526116. Epub 2019 Feb 11. PMID:30808749 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Cytochrome c oxidase 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA