6ojb | pdb_00006ojb

Crystal Structure of Aspergillus fumigatus Ega3 complex with galactosamine

spinqualitylabelsall models 6ojb, resolution 2.09Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ojb is a 1 chain structure with sequence from Aspergillus fumigatus Af293. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.093Å Ligands: BMA, MAN, NAG, X6X Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q4WX16_ASPFU Publication Abstract from PubMed Aspergillus fumigatus is an opportunistic fungal pathogen that causes both chronic and acute invasive infections. Galactosaminogalactan (GAG) is an integral component of the A. fumigatus biofilm matrix and a key virulence factor. GAG is a heterogeneous linear alpha-1,4-linked exopolysaccharide of galactose and GalNAc that is partially deacetylated after secretion. A cluster of five co-expressed genes has been linked to GAG biosynthesis and modification. One gene in this cluster, ega3, is annotated as encoding a putative alpha-1,4-galactosaminidase belonging to glycoside hydrolase family 114 (GH114). Herein, we show that recombinant Ega3 is an active glycoside hydrolase that disrupts GAG-dependent A. fumigatus and Pel polysaccharide-dependent Pseudomonas aeruginosa biofilms at nanomolar concentrations. Using MS and functional assays, we demonstrate that Ega3 is an endo-acting alpha-1,4-galactosaminidase whose activity depends on the conserved acidic residues, Asp-189 and Glu-247. X-ray crystallographic structural analysis of the apo Ega3 and an Ega3-galactosamine complex, at 1.76 and 2.09 A resolutions, revealed a modified (beta/alpha)8-fold with a deep electronegative cleft, which upon ligand binding is capped to form a tunnel. Our structural analysis coupled with in silico docking studies also uncovered the molecular determinants for galactosamine specificity and substrate binding at the -2 to +1 binding subsites. The findings in this study increase the structural and mechanistic understanding of the GH114 family, which has >600 members encoded by plant and opportunistic human pathogens, as well as in industrially used bacteria and fungi. Ega3 from the fungal pathogen Aspergillus fumigatus is an endo-alpha-1,4-galactosaminidase that disrupts microbial biofilms.,Bamford NC, Le Mauff F, Subramanian AS, Yip P, Millan C, Zhang Y, Zacharias C, Forman A, Nitz M, Codee JDC, Uson I, Sheppard DC, Howell PL J Biol Chem. 2019 Sep 13;294(37):13833-13849. doi: 10.1074/jbc.RA119.009910. Epub, 2019 Aug 15. PMID:31416836 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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