6v0t | pdb_00006v0t

Crystal Structure of Catalytic Subunit of Bovine Pyruvate Dehydrogenase Phosphatase 1 - Catalytic Domain

spinqualitylabelsall models 6v0t, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6v0t is a 1 chain structure with sequence from Bos taurus. This structure supersedes the now removed PDB entry 3n3c. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.1Å Ligands: MN, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PDP1_BOVIN Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex. Publication Abstract from PubMed Mammalian pyruvate dehydrogenase (PDH) activity is tightly regulated by phosphorylation and dephosphorylation, which is catalyzed by PDH kinase isomers and PDH phosphatase isomers, respectively. PDH phosphatase isomer 1 (PDP1) is a heterodimer consisting of a catalytic subunit (PDP1c) and a regulatory subunit (PDP1r). Here, the crystal structure of bovine PDP1c determined at 2.1 A resolution is reported. The crystals belonged to space group P3(2)21, with unit-cell parameters a = b = 75.3, c = 173.2 A. The structure was solved by molecular-replacement methods and refined to a final R factor of 21.9% (R(free) = 24.7%). The final model consists of 402 of a possible 467 amino-acid residues of the PDP1c monomer, two Mn(2+) ions in the active site, an additional Mn(2+) ion coordinated by His410 and His414, two MnSO(4) ion pairs at special positions near the crystallographic twofold symmetry axis and 226 water molecules. Several new features of the PDP1c structure are revealed. The requirements are described and plausible bases are deduced for the interaction of PDP1c with PDP1r and other components of the pyruvate dehydrogenase complex. Crystal structure of the catalytic subunit of bovine pyruvate dehydrogenase phosphatase.,Guo Y, Qiu W, Roche TE, Hackert ML Acta Crystallogr F Struct Biol Commun. 2020 Jul 1;76(Pt 7):292-301. doi: , 10.1107/S2053230X20007943. Epub 2020 Jul 1. PMID:32627744 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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