6xjo | pdb_00006xjo
Human atlastin-3 (residues 1-334) bound to GDP-Mg2+ exhibits an ordered amino terminus
Structural highlights
DiseaseATLA3_HUMAN Hereditary sensory and autonomic neuropathy type 1. The disease is caused by mutations affecting the gene represented in this entry. FunctionATLA3_HUMAN GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis.[1] [2] Publication Abstract from PubMedAtlastin (ATL) GTPases catalyze homotypic membrane fusion of the peripheral endoplasmic reticulum (ER). GTP-hydrolysis-driven conformational changes and membrane tethering are prerequisites for proper membrane fusion. However, the molecular basis for regulation of these processes is poorly understood. Here we establish intrinsic and extrinsic modes of ATL1 regulation that involve the N-terminal hypervariable region (HVR) of ATLs. Crystal structures of ATL1 and ATL3 exhibit the HVR as a distinct, isoform-specific structural feature. Characterizing the functional role of ATL1's HVR uncovered its positive effect on membrane tethering and on ATL1's cellular function. The HVR is post-translationally regulated through phosphorylation-dependent modification. A kinase screen identified candidates that modify the HVR site specifically, corresponding to the modifications on ATL1 detected in cells. This work reveals how the HVR contributes to efficient and potentially regulated activity of ATLs, laying the foundation for the identification of cellular effectors of ATL-mediated membrane processes. The hypervariable region of atlastin-1 is a site for intrinsic and extrinsic regulation.,Kelly CM, Byrnes LJ, Neela N, Sondermann H, O'Donnell JP J Cell Biol. 2021 Nov 1;220(11). pii: 212648. doi: 10.1083/jcb.202104128. Epub, 2021 Sep 21. PMID:34546351[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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