7khu | pdb_00007khu

Crystal structure of the carbohydrate-binding domain VP8* of human P[4] rotavirus strain BM5265 in complex with LNDFH I

spinqualitylabelsall models 7khu, resolution 2.54Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7khu is a 2 chain structure with sequence from Human rotavirus A. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.54Å Ligands: BGC, FUC, GAL, NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function E9BVI9_9VIRU Publication Abstract from PubMed Group A rotaviruses cause severe gastroenteritis in infants and young children worldwide, with P[II] genogroup rotaviruses (RVs) responsible for >90% of global cases. RVs have diverse host ranges in different human and animal populations determined by host histo-blood group antigen (HBGA) receptor polymorphism, but details governing diversity, host ranges, and species barriers remain elusive. In this study, crystal structures of complexes of the major P[II] genogroup P[4] and P[8] genotype RV VP8* receptor-binding domains together with Lewis epitope-containing LNDFH I glycans in combination with VP8* receptor-glycan ligand affinity measurements based on NMR titration experiments revealed the structural basis for RV genotype-specific switching between betabeta and betaalpha HBGA receptor-binding sites that determine RV host ranges. The data support the hypothesis that P[II] RV evolution progressed from animals to humans under the selection of type 1 HBGAs guided by stepwise host synthesis of type 1 ABH and Lewis HBGAs. The results help explain disease burden, species barriers, epidemiology, and limited efficacy of current RV vaccines in developing countries. The structural data has the potential to impact the design of future vaccine strategies against RV gastroenteritis. Structural basis of P[II] rotavirus evolution and host ranges under selection of histo-blood group antigens.,Xu S, McGinnis KR, Liu Y, Huang P, Tan M, Stuckert MR, Burnside RE, Jacob EG, Ni S, Jiang X, Kennedy MA Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107963118. doi:, 10.1073/pnas.2107963118. PMID:34475219 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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