7rdb | pdb_00007rdb
Crystal structure of Tspan15 large extracellular loop (Tspan15 LEL)
Structural highlights
FunctionTSN15_HUMAN Regulates maturation and trafficking of the transmembrane metalloprotease ADAM10 (PubMed:26686862, PubMed:30463011). Promotes ADAM10-mediated cleavage of CDH2 (By similarity). Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity (PubMed:26686862).[UniProtKB:F7BWT7][1] [2] Publication Abstract from PubMedTetraspanins are four-pass transmembrane proteins that function by regulating trafficking of partner proteins and organizing signaling complexes in the membrane. Tspan15, one of a six-member TspanC8 subfamily, forms a complex that regulates the trafficking, maturation, and substrate selectivity of the transmembrane protease ADAM10, an essential enzyme in mammalian physiology that cleaves a wide variety of membrane-anchored substrates, including Notch receptors, amyloid precursor protein, cadherins, and growth factors. We present here crystal structures of the Tspan15 large extracellular loop (LEL) required for functional association with ADAM10 both in isolation and in complex with the Fab fragment of an anti-Tspan15 antibody. Comparison of the Tspan15 LEL with other tetraspanin LEL structures shows that a core helical framework buttresses a variable region that structurally diverges among LELs. Using co-immunoprecipitation and a cellular N-cadherin cleavage assay, we identify a site on Tspan15 required for both ADAM10 binding and promoting substrate cleavage. Crystal structure of the Tspan15 LEL domain reveals a conserved ADAM10 binding site.,Lipper CH, Gabriel KH, Seegar TCM, Durr KL, Tomlinson MG, Blacklow SC Structure. 2021 Nov 2. pii: S0969-2126(21)00375-0. doi:, 10.1016/j.str.2021.10.007. PMID:34739841[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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