7sf0 | pdb_00007sf0

Crystal structure of Vaccinia Virus decapping enzyme D9 in complex with trinucleotide substrate

spinqualitylabelsall models 7sf0, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7sf0 is a 1 chain structure with sequence from Vaccinia virus Western Reserve. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.9500045Å Ligands: 8NK, MG, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D9_VACCW Publication Abstract from PubMed Poxviruses encode decapping enzymes that remove the protective 5' cap from both host and viral mRNAs to commit transcripts for decay by the cellular exonuclease Xrn1. Decapping by these enzymes is critical for poxvirus pathogenicity by means of simultaneously suppressing host protein synthesis and limiting the accumulation of viral double-stranded RNA (dsRNA), a trigger for antiviral responses. Here we present a high-resolution structural view of the vaccinia virus decapping enzyme D9. This Nudix enzyme contains a domain organization different from other decapping enzymes in which a three-helix bundle is inserted into the catalytic Nudix domain. The 5' mRNA cap is positioned in a bipartite active site at the interface of the two domains. Specificity for the methylated guanosine cap is achieved by stacking between conserved aromatic residues in a manner similar to that observed in canonical cap-binding proteins VP39, eIF4E, and CBP20, and distinct from eukaryotic decapping enzyme Dcp2. Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysis.,Peters JK, Tibble RW, Warminski M, Jemielity J, Gross JD Structure. 2022 Mar 2. pii: S0969-2126(22)00048-X. doi:, 10.1016/j.str.2022.02.012. PMID:35290794 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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