5me3 | pdb_00005me3

Structure of the Scc2 C-terminus

spinqualitylabelsall models 5me3, resolution 2.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5me3 is a 6 chain structure with sequence from Eremothecium gossypii ATCC 10895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.85Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SCC2_ASHGO Plays a structural role in chromatin. Probably involved in sister chromatid cohesion, possibly by interacting with the cohesin complex (By similarity). Publication Abstract from PubMed The functions of cohesin are central to genome integrity, chromosome organization and transcription regulation through its prevention of premature sister-chromatid separation and the formation of DNA loops. The loading of cohesin onto chromatin depends on the Scc2-Scc4 complex; however, little is known about how it stimulates the cohesion-loading activity. Here we determine the large 'hook' structure of Scc2 responsible for catalysing cohesin loading. We identify key Scc2 surfaces that are crucial for cohesin loading in vivo. With the aid of previously determined structures and homology modelling, we derive a pseudo-atomic structure of the full-length Scc2-Scc4 complex. Finally, using recombinantly purified Scc2-Scc4 and cohesin, we performed crosslinking mass spectrometry and interaction assays that suggest Scc2-Scc4 uses its modular structure to make multiple contacts with cohesin. Structure of the cohesin loader Scc2.,Chao WC, Murayama Y, Munoz S, Jones AW, Wade BO, Purkiss AG, Hu XW, Borg A, Snijders AP, Uhlmann F, Singleton MR Nat Commun. 2017 Jan 6;8:13952. doi: 10.1038/ncomms13952. PMID:28059076 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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