5mis | pdb_00005mis

Crystal Structure of Lactococcus lactis Thioredoxin Reductase Exposed to Visible Light (180 min)

spinqualitylabelsall models 5mis, resolution 1.81Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5mis is a 1 chain structure with sequence from Lactococcus cremoris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.81Å Ligands: FAD, NAP, PEG, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A2RLJ5_LACLM Publication Abstract from PubMed The NADPH-dependent homodimeric flavoenzyme thioredoxin reductase (TrxR) provides reducing equivalents to thioredoxin, a key regulator of various cellular redox processes. Crystal structures of photo-inactivated thioredoxin reductase (TrxR) from the Gram-positive bacterium Lactococcus lactis have been determined. These structures reveal novel molecular features that provide further insight into the mechanisms behind the sensitivity of this enzyme toward visible light. We propose that a pocket on the si-face of the isoalloxazine ring accommodates oxygen that reacts with photo-excited FAD generating superoxide and a flavin radical that oxidize the isoalloxazine ring C7alpha methyl group and a nearby tyrosine residue. This tyrosine and key residues surrounding the oxygen pocket are conserved in enzymes from related bacteria, including pathogens such as Staphylococcus aureus. Photo-sensitivity may thus be a widespread feature among bacterial TrxR with the described characteristics, which affords applications in clinical photo-therapy of drug-resistant bacteria. The structure of Lactococcus lactis thioredoxin reductase reveals molecular features of photo-oxidative damage.,Skjoldager N, Blanner Bang M, Rykaer M, Bjornberg O, Davies MJ, Svensson B, Harris P, Hagglund P Sci Rep. 2017 Apr 11;7:46282. doi: 10.1038/srep46282. PMID:28397795 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Thioredoxin reductase 3D structures

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