8d2j | pdb_00008d2j

A novel insecticidal protein from ferns IPD113_Cow.

spinqualitylabelsall models 8d2j, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8d2j is a 4 chain structure with sequence from Leptochilus wrightii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.1Å Ligands: GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Lepidopterans affect crop production worldwide. The use of transgenes encoding insecticidal proteins from Bacillus thuringiensis (Bt) in crop plants is a well-established technology that enhances protection against lepidopteran larvae. Concern about widespread field-evolved resistance to Bt proteins has highlighted an urgent need for new insecticidal proteins with different modes or sites of action. We discovered a new family of insecticidal proteins from ferns. The prototype protein from Pteris species (Order Polypodiales) and variants from two other orders of ferns, Schizaeales and Ophioglossales, were effective against important lepidopteran pests of maize and soybean in diet-based assays. Transgenic maize and soybean plants producing these proteins were more resistant to insect damage than controls. We report here the crystal structure of a variant of the prototype protein to 1.98 A resolution. Remarkably, despite being derived from plants, the structure resembles the 3-domain Cry proteins from Bt but has only two out of three of their characteristic domains, lacking the C-terminal domain which is typically required for their activities. Two of the fern proteins were effective against strains of fall armyworm that were resistant to Bt 3-domain Cry proteins Cry1Fa or Cry2A.127. This therefore represents a novel family of insecticidal proteins that have the potential to provide future tools for pest control. Novel insecticidal proteins from ferns resemble insecticidal proteins from Bacillus thuringiensis.,Wei JZ, Lum A, Schepers E, Liu L, Weston RT, McGinness BS, Heckert MJ, Xie W, Kassa A, Bruck D, Rauscher G, Kapka-Kitzman D, Mathis JP, Zhao JZ, Sethi A, Barry J, Lu AL, Brugliera F, Lee EL, van derWeerden NL, Eswar N, Maher MJ, Anderson MA Proc Natl Acad Sci U S A. 2023 Oct 31;120(44):e2306177120. doi: , 10.1073/pnas.2306177120. Epub 2023 Oct 23. PMID:37871210 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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