3x3m | pdb_00003x3m

Crystal structure of EccB1 of Mycobacterium tuberculosis in spacegroup P212121

spinqualitylabelsall models 3x3m, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3x3m is a 1 chain structure with sequence from Mycobacterium tuberculosis H37Rv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.9Å Ligands: CA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ECCB1_MYCTU Part of the ESX-1 specialized secretion system, which delivers several virulence factors to host cells during infection, including the key virulence factors EsxA (ESAT-6) and EsxB (CFP-10). Publication Abstract from PubMed Pathogenic mycobacteria transport virulence factors across their complex cell wall via a type VII secretion system (T7SS)/early secreted antigenic target-6 of kDa secretion system (ESX). ESX conserved component (Ecc) B, a core component of the T7SS architecture, is predicted to be a membrane bound protein, but little is known about its structure and function. Here, we characterize EccB1, showing that it is an ATPase with no sequence or structural homology to other ATPases located in the cell envelope of Mycobacterium tuberculosis H37Rv. We obtained the crystal structure of an EccB1-DeltaN72 truncated transmembrane helix and performed modeling and ATP docking studies, showing that EccB1 likely exists as a hexamer. Sequence alignment and ATPase activity determination of EccB1 homologues indicated the presence of 3 conserved motifs in the N- and C-terminals of EccB1-DeltaN72 that assemble together between 2 membrane proximal domains of the EccB1-DeltaN72 monomer. Models of the EccB1 hexamer show that 2 of the conserved motifs are involved in ATPase activity and form an ATP binding pocket located on the surface of 2 adjacent molecules. Our results suggest that EccB may act as the energy provider in the transport of T7SS virulence factors and may be involved in the formation of a channel across the mycomembrane.-Zhang, X.-L., Li, D.-F., Fleming, J., Wang, L.-W., Zhou, Y., Wang, D.-C., Zhang, X.-E., Bi, L.-J. Core component EccB1 of the Mycobacterium tuberculosis type VII secretion system is a periplasmic ATPase. Core component EccB1 of the Mycobacterium tuberculosis type VII secretion system is a periplasmic ATPase.,Zhang XL, Li DF, Fleming J, Wang LW, Zhou Y, Wang DC, Zhang XE, Bi LJ FASEB J. 2015 Dec;29(12):4804-14. doi: 10.1096/fj.15-270843. Epub 2015 Sep 22. PMID:26396239 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also 3D structures of ubiquitin

Proteopedia Page Contributors and Editors (what is this?)

OCA