4fbg | pdb_00004fbg

Crystal structure of Treponema denticola trans-2-enoyl-CoA reductase in complex with NAD

spinqualitylabelsall models 4fbg, resolution 3.02Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4fbg is a 16 chain structure with sequence from Treponema denticola ATCC 35405. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.02Å Ligands: MSE, NAD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function FABV_TREDE Involved in the fatty acid synthesis (FAS II). Catalyzes the reduction of the carbon-carbon double bond of crotonyl-CoA to yield butyryl-CoA. In vitro it can also use hexenoyl-CoA and dodecenoyl-CoA as substrates (PubMed:22906002). Publication Abstract from PubMed Trans-2-enoyl-CoA reductases (TERs, EC 1.3.1.44), which specifically catalyze the reduction of crotonyl-CoA to butyryl-CoA using NADH as cofactor, have recently been applied in the design of robust synthetic pathways to produce 1-butanol as a biofuel. We report here the characterization of a TER homologue in Clostridium acetobutylicum (CaTER), the structures of CaTER in apo form and in complexes with NADH and NAD+, and the structure of Treponema denticola TER (TdTER) in complex with NAD+. Structural and sequence comparisons show that CaTER and TdTER share about 45% overall sequence identity and high structural similarities with the FabV class enoyl-acyl carrier protein reductases in the bacterial fatty acid synthesis pathway, suggesting that both types of enzymes belong to the same family. CaTER and TdTER function as monomers and consist of a cofactor-binding domain and a substrate-binding domain with the catalytic active site located at the interface of the two domains. Structural analyses of CaTER together with mutagenesis and biochemical data indicate that the conserved Glu75 determines the cofactor specificity, and the conserved Tyr225, Tyr235, and Lys244 play critical roles in catalysis. Upon cofactor binding, the substrate-binding loop changes from an open conformation to a closed conformation, narrowing a hydrophobic channel to the catalytic site. A modeling study shows that the hydrophobic channel is optimal in both width and length for the binding of crotonyl-CoA. These results provide molecular bases for the high substrate specificity and the catalytic mechanism of TERs. Structures of trans-2-enoyl-CoA reductases from Clostridium acetobutulicum and Treponema denticola: insights into the substrate specificity and the catalytic mechanism.,Hu K, Zhao M, Zhang T, Zha M, Zhong C, Jiang Y, Ding J Biochem J. 2012 Oct 11. PMID:23050861 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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