2ji6 | pdb_00002ji6
X-RAY STRUCTURE OF OXALYL-COA DECARBOXYLASE IN COMPLEX WITH 3-DEAZA-THDP AND OXALYL-COA
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Overview
Despite more than five decades of extensive studies of thiamin diphosphate, (ThDP) enzymes, there remain many uncertainties as to how these enzymes, achieve their rate enhancements. Here, we present a clear picture of, catalysis for the simple nonoxidative decarboxylase, oxalyl-coenzyme A, (CoA) decarboxylase, based on crystallographic snapshots along the, catalytic cycle and kinetic data on active site mutants. First, we provide, crystallographic evidence that, upon binding of oxalyl-CoA, the C-terminal, 13 residues fold over the substrate, aligning the substrate alpha-carbon, for attack by the ThDP-C2 atom. The second structure presented shows a, covalent reaction intermediate after decarboxylation, interpreted as being, nonplanar. Finally, the structure of a product complex is ... [(full description)]
About this Structure
2JI6 is a [Single protein] structure of sequence from [Oxalobacter formigenes] with MG, TPW, ADP, OXK, PGE and B3P as [ligands]. Active as [[1]], with EC number [4.1.1.8]. Full crystallographic information is available from [OCA].
Reference
Crystallographic snapshots of oxalyl-CoA decarboxylase give insights into catalysis by nonoxidative ThDP-dependent decarboxylases., Berthold CL, Toyota CG, Moussatche P, Wood MD, Leeper F, Richards NG, Lindqvist Y, Structure. 2007 Jul;15(7):853-61. PMID:17637344
Page seeded by OCA on Mon Oct 29 18:23:56 2007
Proteopedia Page Contributors and Editors (what is this?)
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- Oxalobacter formigenes
- Single protein
- Berthold, C.L.
- Leeper, F.
- Lindqvist, Y.
- Moussatche, P.
- Richards, N.G.J.
- Toyota, C.G.
- Wood, M.D.
- ADP
- B3P
- MG
- OXK
- PGE
- TPW
- Decarboxylase
- Flavoprotein
- Lyase
- Non- oxidative decarboxylase
- Oxalate degradation
- Substrate complex
- Thiamin diphosphate-dependent
- Thiamine pyrophosphate
