4pws | pdb_00004pws

Crystal structure of secreted proline rich antigen MTC28 (Rv0040c) at 2.15 A with bound chloride from Mycobacterium tuberculosis

spinqualitylabelsall models 4pws, resolution 2.15Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4pws is a 1 chain structure with sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.15Å Ligands: CL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PR28_MYCTU Publication Abstract from PubMed Secretary proteins of Mycobacterium tuberculosis are key players of the mycobacterial infection pathway. MTC28 is a 28-kDa proline-rich secretary antigen of Mycobacterium tuberculosis and is only conserved in pathogenic strains of mycobacteria. Here we report the crystal structure of MTC28 at 2.8- and 2.15-A resolutions for the structure-based epitope design. MTC28 shares a "mog1p"-fold consisting of seven antiparallel beta strands stacked between alpha helices. Five probable epitopes have been located on a solvent-accessible flexible region by computational analysis of the structure of MTC28. Simultaneously, the protein is digested with trypsin and the resulting fragments are purified by HPLC. Such 10 purified peptide fragments are screened against sera from patients infected with pulmonary tuberculosis (PTB). Two of these 10 fragments, namely (127)ALDITLPMPPR(137) and (138)WTQVPDPNVPDAFVVIADR(156),are found to be major immunogenic epitopes that are localized on the outer surface of the protein molecule and are part of a single continuous epitope that have been predicted in silico Mutagenesis and antibody inhibition studies are in accordance with the results obtained from epitope mapping. Structure-based Epitope Mapping of Mycobacterium tuberculosis Secretary Antigen MTC28.,Kundu P, Biswas R, Mukherjee S, Reinhard L, Dutta A, Mueller-Dieckmann J, Weiss MS, Pal NK, Das AK J Biol Chem. 2016 Jul 1;291(27):13943-54. doi: 10.1074/jbc.M116.726422. Epub 2016, May 4. PMID:27189947 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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