5cj8 | pdb_00005cj8

Human Osteoclast Associated Receptor (OSCAR) extracellular domain

spinqualitylabelsall models 5cj8, resolution 2.02Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5cj8 is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.015Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function OSCAR_HUMAN Regulator of osteoclastogenesis which plays an important bone-specific function in osteoclast differentiation. Publication Abstract from PubMed Human osteoclast-associated receptor (OSCAR) is an immunoglobulin (Ig)-like collagen receptor that is up-regulated on osteoclasts during osteoclastogenesis and is expressed in a range of myeloid cells. As a member of the leukocyte receptor complex family of proteins, OSCAR shares a high degree of sequence and structural homology with other collagen receptors of this family, including glycoprotein VI, leukocyte-associated Ig-like receptor-1, and leukocyte Ig-like receptor B4, but recognizes a unique collagen sequence. Here, we present the crystal structures of OSCAR in its free form and in complex with a triple-helical collagen-like peptide (CLP). These structures reveal that the CLP peptide binds only one of the two Ig-like domains, the membrane-proximal domain (domain 2) of OSCAR, with the middle and trailing chain burying a total of 661 A2 of solvent-accessible collagen surface. This binding mode is facilitated by the unusual topography of the OSCAR protein, which displays an obtuse interdomain angle and a rotation of domain 2 relative to the membrane-distal domain 1. Moreover, the binding of the CLP to OSCAR appears to be mediated largely by tyrosine residues and conformational changes at a shallow Phe pocket. Furthermore, we investigated CLP peptides as inhibitors of osteoclastogenesis and found that a peptide length of 40 amino acids is required to ensure adequate inhibition of osteoclastogenesis in vitro. These findings provide valuable structural insights into the mode of collagen recognition by OSCAR and into the use of synthetic peptide matrikines for osteoclastogenesis inhibition. Structural basis of collagen recognition by human osteoclast-associated receptor and design of osteoclastogenesis inhibitors.,Haywood J, Qi J, Chen CC, Lu G, Liu Y, Yan J, Shi Y, Gao GF Proc Natl Acad Sci U S A. 2016 Jan 7. pii: 201522572. PMID:26744311 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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