5n2c | pdb_00005n2c

Crystal structure of the peptidoglycan-associated lipoprotein from Burkholderia cenocepacia

spinqualitylabelsall models 5n2c, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5n2c is a 3 chain structure with sequence from Burkholderia cenocepacia J2315. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: ACT, CXS, EDO, OXL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B4EDC1_BURCJ Publication Abstract from PubMed Structure-based epitope prediction drives the design of diagnostic peptidic probes to reveal specific antibodies elicited in response to infections. We previously identified a highly immunoreactive epitope from the peptidoglycan-associated lipoprotein (Pal) antigen from Burkholderia pseudomallei, which could also diagnose Burkholderia cepacia infections. Here, considering the high phylogenetic conservation within Burkholderia species, we ask whether cross-reactivity can be reciprocally displayed by the synthetic epitope from B. cenocepacia. We perform comparative analyses of the conformational preferences and diagnostic performances of the corresponding epitopes from the two Burkholderia species when presented in the context of the full-length proteins or as isolated peptides. The effects of conformation on the diagnostic potential and cross-reactivity of Pal peptide epitopes are rationalized on the basis of the 1.8 A crystal structure of B. cenocepacia Pal and through computational analyses. Our results are discussed in the context of designing new diagnostic molecules for the early detection of infectious diseases. Designing Probes for Immunodiagnostics: Structural Insights into an Epitope Targeting Burkholderia Infections.,Capelli R, Matterazzo E, Amabili M, Peri C, Gori A, Gagni P, Chiari M, Lertmemongkolchai G, Cretich M, Bolognesi M, Colombo G, Gourlay LJ ACS Infect Dis. 2017 Jul 21. doi: 10.1021/acsinfecdis.7b00080. PMID:28707874 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Pal

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